Mechanism of Agrobacterium mediated gene transfer
The last article described the Agrobacterium-mediated gene transfer in plants via Ti-plasmid, in this article the mechanism of transfer of T-DNA is described in detail. The process of integration of…
Mitesh Khisti
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Agrobacterium-mediated transformation of plants
As discussed in previous articles, the Agrobacterium-mediated transformation of plants is the most widely used technique for plant transformation. In this article, we will discuss the technique in detail. Agrobacterium…
ABC model of flower development in plants
Flower development in plants is governed by flowering genes. Flowering genes in plants are of two types, floral meristem identity genes, and floral organs identity genes. The floral organ identity…
Genetically Modified Crops
Since the domestication of the plants, efforts are made all over the world to increase the yield of plant products and obtain different plant products via conserving the useful traits…
Plant Hormones
In complex organisms like mammals to keep all the cellular as well as physiological processes going, communication between cells, tissues and organs is of paramount importance. This communication is achieved…
Western Blotting
Western blotting is a molecular biology technique widely used to detect the protein of interest from a pool of many proteins in a cell. Western blotting is also called protein…
Quantitative Real-Time PCR (qRT-PCR)
For most Molecular biology studies, it is crucial to quantify the product obtained after a PCR. Many methods have been discovered for this quantification, characterized under two categories: homogenous and…
Identifying Protein-Protein interactions
Each organism on this Earth has a wide range of proteins that drive the various biological processes and biochemical reactions occurring within the cell. These reactions and processes sometimes require…
Nested Polymerase Chain Reaction (nPCR)
Polymerase Chain Reaction (PCR) is a technique used to amplify the DNA fragment of interest. From discovering the PCR, PCR has been a great tool in molecular biology with a…
Agarose Gel Electrophoresis
Electrophoresis is a term referred to as the movement of charged molecules in an electric field. Agarose gel electrophoresis is commonly used to separate/purify larger DNA fragments. This technique is…
