Introduction: Nucleotides are the building blocks of DNA and RNA, essential for all living organisms. They play critical roles in various cellular processes, including energy transfer, signaling, and gene expression. While nucleotides can be obtained…
Introduction: In the vast world of molecular biology, understanding protein-protein interactions is crucial for deciphering the intricate workings of biological systems. The Yeast Two-Hybrid (Y2H) assay has emerged as a powerful tool to explore and…
Introduction: Restriction enzyme digestion of DNA is a fundamental technique in molecular biology that has revolutionized genetic research and biotechnology. These enzymes, derived from bacteria and other organisms, play a crucial role in DNA manipulation…
Each organism genome contains 1000s of genes, each gene (or DNA) is transcribed into RNA, which is eventually translated into protein. Blotting technique for the detection of RNA is known as Northern blotting. To study…
Western blotting is a molecular biology technique widely used to detect the protein of interest from a pool of many proteins in a cell. Western blotting is also called protein immunoblot as it uses antibodies…
For most Molecular biology studies, it is crucial to quantify the product obtained after a PCR. Many methods have been discovered for this quantification, characterized under two categories: homogenous and heterogeneous methods. Heterogenous methods involve…
Each organism on this Earth has a wide range of proteins that drive the various biological processes and biochemical reactions occurring within the cell. These reactions and processes sometimes require the interaction between two proteins;…
Polymerase Chain Reaction (PCR) is a technique used to amplify the DNA fragment of interest. From discovering the PCR, PCR has been a great tool in molecular biology with a wide range of applications in…
In this era of the COVID-19 pandemic, every person, be it from a scientific or non-scientific background, knows the word “PCR”. But what does that mean? Why is it used and the principle behind using…
Electrophoresis is a term referred to as the movement of charged molecules in an electric field. Agarose gel electrophoresis is commonly used to separate/purify larger DNA fragments. This technique is used to separate DNA fragments…
