Western Blotting
Western blotting is a molecular biology technique widely used to detect the protein of interest from a pool of many proteins in a cell. Western blotting is also called protein immunoblot as it uses antibodies…
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Quantitative Real-Time PCR (qRT-PCR)
For most Molecular biology studies, it is crucial to quantify the product obtained after a PCR. Many methods have been discovered for this quantification, characterized under two categories: homogenous and heterogeneous methods. Heterogenous methods involve…
Touchdown PCR
Polymerase Chain Reaction (PCR) is a technique used to amplify the DNA fragment of interest. From discovering the PCR, PCR has been a great tool in molecular biology with a wide range of applications in…
Nested Polymerase Chain Reaction (nPCR)
Polymerase Chain Reaction (PCR) is a technique used to amplify the DNA fragment of interest. From discovering the PCR, PCR has been a great tool in molecular biology with a wide range of applications in…
Agarose Gel Electrophoresis
Electrophoresis is a term referred to as the movement of charged molecules in an electric field. Agarose gel electrophoresis is commonly used to separate/purify larger DNA fragments. This technique is used to separate DNA fragments…
Identification of Molecular Markers
During evolution, many variations have been accumulated in different plant species. These variations are in the form of mutations such as insertion, deletion, inversion and substitution. These variations give rise to diversity. Diversity is considered…
ELISA (Enzyme-Linked Immunosorbent Assay)
ELISA, which stands for Enzyme-Linked Immunosorbent Assay, is a widely used technique in immunology to detect the presence of various antibodies or antigens in a sample. ELISA techniques have proved their supremacy over Radioimmunoassay (RIA)…
SARS-CoV-2 detection by RT-PCR:
Covid-19 is a global pandemic caused due to a virus known as SARS-CoV-2. This disease is impacting the world to the extent that is not imagined by any of us. The key behind controlling the…
